solid phase sandwich elisa kit Search Results


solid phase elisa test quickvue influenza a kit  (Quidel)
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Quidel solid phase elisa test quickvue influenza a kit
Solid Phase Elisa Test Quickvue Influenza A Kit, supplied by Quidel, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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solid phase enzyme-linked immunosorbent assay kit demeditec shbg elisa  (DEMEDITEC Diagnostics GmbH)
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DEMEDITEC Diagnostics GmbH solid phase enzyme-linked immunosorbent assay kit demeditec shbg elisa
Solid Phase Enzyme Linked Immunosorbent Assay Kit Demeditec Shbg Elisa, supplied by DEMEDITEC Diagnostics GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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dual-antibody solid-phase immunoassays elisa development kit  (PeproTech)
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PeproTech dual-antibody solid-phase immunoassays elisa development kit
β-FNAs effect on <t>LPS-induced</t> <t>CCL2</t> expression in the whole brain and brain regions (hippocampus, prefrontal cortex, and cerebellum/brain stem) of male and female C57BL/6J mice. Mice ( n = 5–6/group) were injected (i.p.) with saline (control), LPS (0.83 mg/kg), LPS followed immediately by β-FNA treatment (50 mg/kg; i.p.; LPS + β-FNA), or LPS followed by β-FNA 10 h post-LPS (LPS + β-FNA 10 h). 24 h post-LPS, mice were terminated followed by tissue collection. Levels of CCL2 of whole brain ( A ), hippocampus ( B ), prefrontal cortex ( C ), and cerebellum/brain stem ( D ) of tissue homogenates were measured by <t>ELISA.</t> Data are reported as mean ± SEM. Two-way ANOVA indicated a significant main effect of treatment ( p < 0.001) on CCL2 levels in the whole brain; but no significant effect of sex ( p = 0.47), nor a significant interaction ( p = 0.81). Two-way ANOVA determined CCL2 in the hippocampus had a no significant main effect of sex ( p = 0.61), treatment ( p = 0.14), or interaction ( p = 0.79). In the prefrontal cortex two-way ANOVA determined CCL2 had a significant main effect of sex ( p < 0.0001), treatment ( p < 0.001), and an interaction ( p < 0.005). Two-way ANOVA determined in the cerebellum/brain stem that CCL2 had a significant main effect of sex ( p < 0.005), treatment ( p < 0.005), and an interaction ( p < 0.02). Pairwise comparisons were assessed using a Fisher’s LSD test; * indicates p < 0.05 vs. saline group; # indicates p < 0.05 vs. LPS group; Δ indicates p < 0.05 vs. males LPS
Dual Antibody Solid Phase Immunoassays Elisa Development Kit, supplied by PeproTech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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immunocomb® avian c. psittaci solid-phase elisa antibody test kit  (Biogal Galed Labs)
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Biogal Galed Labs immunocomb® avian c. psittaci solid-phase elisa antibody test kit
β-FNAs effect on <t>LPS-induced</t> <t>CCL2</t> expression in the whole brain and brain regions (hippocampus, prefrontal cortex, and cerebellum/brain stem) of male and female C57BL/6J mice. Mice ( n = 5–6/group) were injected (i.p.) with saline (control), LPS (0.83 mg/kg), LPS followed immediately by β-FNA treatment (50 mg/kg; i.p.; LPS + β-FNA), or LPS followed by β-FNA 10 h post-LPS (LPS + β-FNA 10 h). 24 h post-LPS, mice were terminated followed by tissue collection. Levels of CCL2 of whole brain ( A ), hippocampus ( B ), prefrontal cortex ( C ), and cerebellum/brain stem ( D ) of tissue homogenates were measured by <t>ELISA.</t> Data are reported as mean ± SEM. Two-way ANOVA indicated a significant main effect of treatment ( p < 0.001) on CCL2 levels in the whole brain; but no significant effect of sex ( p = 0.47), nor a significant interaction ( p = 0.81). Two-way ANOVA determined CCL2 in the hippocampus had a no significant main effect of sex ( p = 0.61), treatment ( p = 0.14), or interaction ( p = 0.79). In the prefrontal cortex two-way ANOVA determined CCL2 had a significant main effect of sex ( p < 0.0001), treatment ( p < 0.001), and an interaction ( p < 0.005). Two-way ANOVA determined in the cerebellum/brain stem that CCL2 had a significant main effect of sex ( p < 0.005), treatment ( p < 0.005), and an interaction ( p < 0.02). Pairwise comparisons were assessed using a Fisher’s LSD test; * indicates p < 0.05 vs. saline group; # indicates p < 0.05 vs. LPS group; Δ indicates p < 0.05 vs. males LPS
Immunocomb® Avian C. Psittaci Solid Phase Elisa Antibody Test Kit, supplied by Biogal Galed Labs, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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solid-phase enzymelinked immunosorbent assay (elisa) kit  (Pharmacia Diagnostics Verwaltungs GmbH)
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Pharmacia Diagnostics Verwaltungs GmbH solid-phase enzymelinked immunosorbent assay (elisa) kit
β-FNAs effect on <t>LPS-induced</t> <t>CCL2</t> expression in the whole brain and brain regions (hippocampus, prefrontal cortex, and cerebellum/brain stem) of male and female C57BL/6J mice. Mice ( n = 5–6/group) were injected (i.p.) with saline (control), LPS (0.83 mg/kg), LPS followed immediately by β-FNA treatment (50 mg/kg; i.p.; LPS + β-FNA), or LPS followed by β-FNA 10 h post-LPS (LPS + β-FNA 10 h). 24 h post-LPS, mice were terminated followed by tissue collection. Levels of CCL2 of whole brain ( A ), hippocampus ( B ), prefrontal cortex ( C ), and cerebellum/brain stem ( D ) of tissue homogenates were measured by <t>ELISA.</t> Data are reported as mean ± SEM. Two-way ANOVA indicated a significant main effect of treatment ( p < 0.001) on CCL2 levels in the whole brain; but no significant effect of sex ( p = 0.47), nor a significant interaction ( p = 0.81). Two-way ANOVA determined CCL2 in the hippocampus had a no significant main effect of sex ( p = 0.61), treatment ( p = 0.14), or interaction ( p = 0.79). In the prefrontal cortex two-way ANOVA determined CCL2 had a significant main effect of sex ( p < 0.0001), treatment ( p < 0.001), and an interaction ( p < 0.005). Two-way ANOVA determined in the cerebellum/brain stem that CCL2 had a significant main effect of sex ( p < 0.005), treatment ( p < 0.005), and an interaction ( p < 0.02). Pairwise comparisons were assessed using a Fisher’s LSD test; * indicates p < 0.05 vs. saline group; # indicates p < 0.05 vs. LPS group; Δ indicates p < 0.05 vs. males LPS
Solid Phase Enzymelinked Immunosorbent Assay (Elisa) Kit, supplied by Pharmacia Diagnostics Verwaltungs GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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solid phase-conjugated sandwich rat elisa kit  (Merck KGaA)
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Merck KGaA solid phase-conjugated sandwich rat elisa kit
β-FNAs effect on <t>LPS-induced</t> <t>CCL2</t> expression in the whole brain and brain regions (hippocampus, prefrontal cortex, and cerebellum/brain stem) of male and female C57BL/6J mice. Mice ( n = 5–6/group) were injected (i.p.) with saline (control), LPS (0.83 mg/kg), LPS followed immediately by β-FNA treatment (50 mg/kg; i.p.; LPS + β-FNA), or LPS followed by β-FNA 10 h post-LPS (LPS + β-FNA 10 h). 24 h post-LPS, mice were terminated followed by tissue collection. Levels of CCL2 of whole brain ( A ), hippocampus ( B ), prefrontal cortex ( C ), and cerebellum/brain stem ( D ) of tissue homogenates were measured by <t>ELISA.</t> Data are reported as mean ± SEM. Two-way ANOVA indicated a significant main effect of treatment ( p < 0.001) on CCL2 levels in the whole brain; but no significant effect of sex ( p = 0.47), nor a significant interaction ( p = 0.81). Two-way ANOVA determined CCL2 in the hippocampus had a no significant main effect of sex ( p = 0.61), treatment ( p = 0.14), or interaction ( p = 0.79). In the prefrontal cortex two-way ANOVA determined CCL2 had a significant main effect of sex ( p < 0.0001), treatment ( p < 0.001), and an interaction ( p < 0.005). Two-way ANOVA determined in the cerebellum/brain stem that CCL2 had a significant main effect of sex ( p < 0.005), treatment ( p < 0.005), and an interaction ( p < 0.02). Pairwise comparisons were assessed using a Fisher’s LSD test; * indicates p < 0.05 vs. saline group; # indicates p < 0.05 vs. LPS group; Δ indicates p < 0.05 vs. males LPS
Solid Phase Conjugated Sandwich Rat Elisa Kit, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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solid phase-conjugated sandwich rat elisa kit - by Bioz Stars, 2026-08
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solid phase competitive enzyme-linked immune sorbent assay (elisa) kit  (DiaSys Inc)
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DiaSys Inc solid phase competitive enzyme-linked immune sorbent assay (elisa) kit
β-FNAs effect on <t>LPS-induced</t> <t>CCL2</t> expression in the whole brain and brain regions (hippocampus, prefrontal cortex, and cerebellum/brain stem) of male and female C57BL/6J mice. Mice ( n = 5–6/group) were injected (i.p.) with saline (control), LPS (0.83 mg/kg), LPS followed immediately by β-FNA treatment (50 mg/kg; i.p.; LPS + β-FNA), or LPS followed by β-FNA 10 h post-LPS (LPS + β-FNA 10 h). 24 h post-LPS, mice were terminated followed by tissue collection. Levels of CCL2 of whole brain ( A ), hippocampus ( B ), prefrontal cortex ( C ), and cerebellum/brain stem ( D ) of tissue homogenates were measured by <t>ELISA.</t> Data are reported as mean ± SEM. Two-way ANOVA indicated a significant main effect of treatment ( p < 0.001) on CCL2 levels in the whole brain; but no significant effect of sex ( p = 0.47), nor a significant interaction ( p = 0.81). Two-way ANOVA determined CCL2 in the hippocampus had a no significant main effect of sex ( p = 0.61), treatment ( p = 0.14), or interaction ( p = 0.79). In the prefrontal cortex two-way ANOVA determined CCL2 had a significant main effect of sex ( p < 0.0001), treatment ( p < 0.001), and an interaction ( p < 0.005). Two-way ANOVA determined in the cerebellum/brain stem that CCL2 had a significant main effect of sex ( p < 0.005), treatment ( p < 0.005), and an interaction ( p < 0.02). Pairwise comparisons were assessed using a Fisher’s LSD test; * indicates p < 0.05 vs. saline group; # indicates p < 0.05 vs. LPS group; Δ indicates p < 0.05 vs. males LPS
Solid Phase Competitive Enzyme Linked Immune Sorbent Assay (Elisa) Kit, supplied by DiaSys Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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solid phase competitive enzyme-linked immune sorbent assay (elisa) kit - by Bioz Stars, 2026-08
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solid phase two-site enzyme immunoassay drg ultrasensitive rat insulin elisa kit  (DRG International)
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DRG International solid phase two-site enzyme immunoassay drg ultrasensitive rat insulin elisa kit
β-FNAs effect on <t>LPS-induced</t> <t>CCL2</t> expression in the whole brain and brain regions (hippocampus, prefrontal cortex, and cerebellum/brain stem) of male and female C57BL/6J mice. Mice ( n = 5–6/group) were injected (i.p.) with saline (control), LPS (0.83 mg/kg), LPS followed immediately by β-FNA treatment (50 mg/kg; i.p.; LPS + β-FNA), or LPS followed by β-FNA 10 h post-LPS (LPS + β-FNA 10 h). 24 h post-LPS, mice were terminated followed by tissue collection. Levels of CCL2 of whole brain ( A ), hippocampus ( B ), prefrontal cortex ( C ), and cerebellum/brain stem ( D ) of tissue homogenates were measured by <t>ELISA.</t> Data are reported as mean ± SEM. Two-way ANOVA indicated a significant main effect of treatment ( p < 0.001) on CCL2 levels in the whole brain; but no significant effect of sex ( p = 0.47), nor a significant interaction ( p = 0.81). Two-way ANOVA determined CCL2 in the hippocampus had a no significant main effect of sex ( p = 0.61), treatment ( p = 0.14), or interaction ( p = 0.79). In the prefrontal cortex two-way ANOVA determined CCL2 had a significant main effect of sex ( p < 0.0001), treatment ( p < 0.001), and an interaction ( p < 0.005). Two-way ANOVA determined in the cerebellum/brain stem that CCL2 had a significant main effect of sex ( p < 0.005), treatment ( p < 0.005), and an interaction ( p < 0.02). Pairwise comparisons were assessed using a Fisher’s LSD test; * indicates p < 0.05 vs. saline group; # indicates p < 0.05 vs. LPS group; Δ indicates p < 0.05 vs. males LPS
Solid Phase Two Site Enzyme Immunoassay Drg Ultrasensitive Rat Insulin Elisa Kit, supplied by DRG International, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mouse keratinocyte chemoattractant (kc) solid phase sandwich elisa kit  (RayBiotech inc)
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RayBiotech inc mouse keratinocyte chemoattractant (kc) solid phase sandwich elisa kit
β-FNAs effect on <t>LPS-induced</t> <t>CCL2</t> expression in the whole brain and brain regions (hippocampus, prefrontal cortex, and cerebellum/brain stem) of male and female C57BL/6J mice. Mice ( n = 5–6/group) were injected (i.p.) with saline (control), LPS (0.83 mg/kg), LPS followed immediately by β-FNA treatment (50 mg/kg; i.p.; LPS + β-FNA), or LPS followed by β-FNA 10 h post-LPS (LPS + β-FNA 10 h). 24 h post-LPS, mice were terminated followed by tissue collection. Levels of CCL2 of whole brain ( A ), hippocampus ( B ), prefrontal cortex ( C ), and cerebellum/brain stem ( D ) of tissue homogenates were measured by <t>ELISA.</t> Data are reported as mean ± SEM. Two-way ANOVA indicated a significant main effect of treatment ( p < 0.001) on CCL2 levels in the whole brain; but no significant effect of sex ( p = 0.47), nor a significant interaction ( p = 0.81). Two-way ANOVA determined CCL2 in the hippocampus had a no significant main effect of sex ( p = 0.61), treatment ( p = 0.14), or interaction ( p = 0.79). In the prefrontal cortex two-way ANOVA determined CCL2 had a significant main effect of sex ( p < 0.0001), treatment ( p < 0.001), and an interaction ( p < 0.005). Two-way ANOVA determined in the cerebellum/brain stem that CCL2 had a significant main effect of sex ( p < 0.005), treatment ( p < 0.005), and an interaction ( p < 0.02). Pairwise comparisons were assessed using a Fisher’s LSD test; * indicates p < 0.05 vs. saline group; # indicates p < 0.05 vs. LPS group; Δ indicates p < 0.05 vs. males LPS
Mouse Keratinocyte Chemoattractant (Kc) Solid Phase Sandwich Elisa Kit, supplied by RayBiotech inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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solid phase enzyme-linked immuno-absorbent assay (elisa) kit  (Enzo Biochem)
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Enzo Biochem solid phase enzyme-linked immuno-absorbent assay (elisa) kit
β-FNAs effect on <t>LPS-induced</t> <t>CCL2</t> expression in the whole brain and brain regions (hippocampus, prefrontal cortex, and cerebellum/brain stem) of male and female C57BL/6J mice. Mice ( n = 5–6/group) were injected (i.p.) with saline (control), LPS (0.83 mg/kg), LPS followed immediately by β-FNA treatment (50 mg/kg; i.p.; LPS + β-FNA), or LPS followed by β-FNA 10 h post-LPS (LPS + β-FNA 10 h). 24 h post-LPS, mice were terminated followed by tissue collection. Levels of CCL2 of whole brain ( A ), hippocampus ( B ), prefrontal cortex ( C ), and cerebellum/brain stem ( D ) of tissue homogenates were measured by <t>ELISA.</t> Data are reported as mean ± SEM. Two-way ANOVA indicated a significant main effect of treatment ( p < 0.001) on CCL2 levels in the whole brain; but no significant effect of sex ( p = 0.47), nor a significant interaction ( p = 0.81). Two-way ANOVA determined CCL2 in the hippocampus had a no significant main effect of sex ( p = 0.61), treatment ( p = 0.14), or interaction ( p = 0.79). In the prefrontal cortex two-way ANOVA determined CCL2 had a significant main effect of sex ( p < 0.0001), treatment ( p < 0.001), and an interaction ( p < 0.005). Two-way ANOVA determined in the cerebellum/brain stem that CCL2 had a significant main effect of sex ( p < 0.005), treatment ( p < 0.005), and an interaction ( p < 0.02). Pairwise comparisons were assessed using a Fisher’s LSD test; * indicates p < 0.05 vs. saline group; # indicates p < 0.05 vs. LPS group; Δ indicates p < 0.05 vs. males LPS
Solid Phase Enzyme Linked Immuno Absorbent Assay (Elisa) Kit, supplied by Enzo Biochem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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solid-phase competitive inhibition elisa kit  (Hawaii Biotech)
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β-FNAs effect on <t>LPS-induced</t> <t>CCL2</t> expression in the whole brain and brain regions (hippocampus, prefrontal cortex, and cerebellum/brain stem) of male and female C57BL/6J mice. Mice ( n = 5–6/group) were injected (i.p.) with saline (control), LPS (0.83 mg/kg), LPS followed immediately by β-FNA treatment (50 mg/kg; i.p.; LPS + β-FNA), or LPS followed by β-FNA 10 h post-LPS (LPS + β-FNA 10 h). 24 h post-LPS, mice were terminated followed by tissue collection. Levels of CCL2 of whole brain ( A ), hippocampus ( B ), prefrontal cortex ( C ), and cerebellum/brain stem ( D ) of tissue homogenates were measured by <t>ELISA.</t> Data are reported as mean ± SEM. Two-way ANOVA indicated a significant main effect of treatment ( p < 0.001) on CCL2 levels in the whole brain; but no significant effect of sex ( p = 0.47), nor a significant interaction ( p = 0.81). Two-way ANOVA determined CCL2 in the hippocampus had a no significant main effect of sex ( p = 0.61), treatment ( p = 0.14), or interaction ( p = 0.79). In the prefrontal cortex two-way ANOVA determined CCL2 had a significant main effect of sex ( p < 0.0001), treatment ( p < 0.001), and an interaction ( p < 0.005). Two-way ANOVA determined in the cerebellum/brain stem that CCL2 had a significant main effect of sex ( p < 0.005), treatment ( p < 0.005), and an interaction ( p < 0.02). Pairwise comparisons were assessed using a Fisher’s LSD test; * indicates p < 0.05 vs. saline group; # indicates p < 0.05 vs. LPS group; Δ indicates p < 0.05 vs. males LPS
Solid Phase Competitive Inhibition Elisa Kit, supplied by Hawaii Biotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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solid-phase enzymelinked immunosorbent assay (elisa) kit no. eia 1864  (DRG Instruments GmbH)
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DRG Instruments GmbH solid-phase enzymelinked immunosorbent assay (elisa) kit no. eia 1864
β-FNAs effect on <t>LPS-induced</t> <t>CCL2</t> expression in the whole brain and brain regions (hippocampus, prefrontal cortex, and cerebellum/brain stem) of male and female C57BL/6J mice. Mice ( n = 5–6/group) were injected (i.p.) with saline (control), LPS (0.83 mg/kg), LPS followed immediately by β-FNA treatment (50 mg/kg; i.p.; LPS + β-FNA), or LPS followed by β-FNA 10 h post-LPS (LPS + β-FNA 10 h). 24 h post-LPS, mice were terminated followed by tissue collection. Levels of CCL2 of whole brain ( A ), hippocampus ( B ), prefrontal cortex ( C ), and cerebellum/brain stem ( D ) of tissue homogenates were measured by <t>ELISA.</t> Data are reported as mean ± SEM. Two-way ANOVA indicated a significant main effect of treatment ( p < 0.001) on CCL2 levels in the whole brain; but no significant effect of sex ( p = 0.47), nor a significant interaction ( p = 0.81). Two-way ANOVA determined CCL2 in the hippocampus had a no significant main effect of sex ( p = 0.61), treatment ( p = 0.14), or interaction ( p = 0.79). In the prefrontal cortex two-way ANOVA determined CCL2 had a significant main effect of sex ( p < 0.0001), treatment ( p < 0.001), and an interaction ( p < 0.005). Two-way ANOVA determined in the cerebellum/brain stem that CCL2 had a significant main effect of sex ( p < 0.005), treatment ( p < 0.005), and an interaction ( p < 0.02). Pairwise comparisons were assessed using a Fisher’s LSD test; * indicates p < 0.05 vs. saline group; # indicates p < 0.05 vs. LPS group; Δ indicates p < 0.05 vs. males LPS
Solid Phase Enzymelinked Immunosorbent Assay (Elisa) Kit No. Eia 1864, supplied by DRG Instruments GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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β-FNAs effect on LPS-induced CCL2 expression in the whole brain and brain regions (hippocampus, prefrontal cortex, and cerebellum/brain stem) of male and female C57BL/6J mice. Mice ( n = 5–6/group) were injected (i.p.) with saline (control), LPS (0.83 mg/kg), LPS followed immediately by β-FNA treatment (50 mg/kg; i.p.; LPS + β-FNA), or LPS followed by β-FNA 10 h post-LPS (LPS + β-FNA 10 h). 24 h post-LPS, mice were terminated followed by tissue collection. Levels of CCL2 of whole brain ( A ), hippocampus ( B ), prefrontal cortex ( C ), and cerebellum/brain stem ( D ) of tissue homogenates were measured by ELISA. Data are reported as mean ± SEM. Two-way ANOVA indicated a significant main effect of treatment ( p < 0.001) on CCL2 levels in the whole brain; but no significant effect of sex ( p = 0.47), nor a significant interaction ( p = 0.81). Two-way ANOVA determined CCL2 in the hippocampus had a no significant main effect of sex ( p = 0.61), treatment ( p = 0.14), or interaction ( p = 0.79). In the prefrontal cortex two-way ANOVA determined CCL2 had a significant main effect of sex ( p < 0.0001), treatment ( p < 0.001), and an interaction ( p < 0.005). Two-way ANOVA determined in the cerebellum/brain stem that CCL2 had a significant main effect of sex ( p < 0.005), treatment ( p < 0.005), and an interaction ( p < 0.02). Pairwise comparisons were assessed using a Fisher’s LSD test; * indicates p < 0.05 vs. saline group; # indicates p < 0.05 vs. LPS group; Δ indicates p < 0.05 vs. males LPS

Journal: Journal of Inflammation (London, England)

Article Title: Anti-inflammatory effects of β-FNA are sex-dependent in a pre-clinical model of LPS-induced inflammation

doi: 10.1186/s12950-023-00328-z

Figure Lengend Snippet: β-FNAs effect on LPS-induced CCL2 expression in the whole brain and brain regions (hippocampus, prefrontal cortex, and cerebellum/brain stem) of male and female C57BL/6J mice. Mice ( n = 5–6/group) were injected (i.p.) with saline (control), LPS (0.83 mg/kg), LPS followed immediately by β-FNA treatment (50 mg/kg; i.p.; LPS + β-FNA), or LPS followed by β-FNA 10 h post-LPS (LPS + β-FNA 10 h). 24 h post-LPS, mice were terminated followed by tissue collection. Levels of CCL2 of whole brain ( A ), hippocampus ( B ), prefrontal cortex ( C ), and cerebellum/brain stem ( D ) of tissue homogenates were measured by ELISA. Data are reported as mean ± SEM. Two-way ANOVA indicated a significant main effect of treatment ( p < 0.001) on CCL2 levels in the whole brain; but no significant effect of sex ( p = 0.47), nor a significant interaction ( p = 0.81). Two-way ANOVA determined CCL2 in the hippocampus had a no significant main effect of sex ( p = 0.61), treatment ( p = 0.14), or interaction ( p = 0.79). In the prefrontal cortex two-way ANOVA determined CCL2 had a significant main effect of sex ( p < 0.0001), treatment ( p < 0.001), and an interaction ( p < 0.005). Two-way ANOVA determined in the cerebellum/brain stem that CCL2 had a significant main effect of sex ( p < 0.005), treatment ( p < 0.005), and an interaction ( p < 0.02). Pairwise comparisons were assessed using a Fisher’s LSD test; * indicates p < 0.05 vs. saline group; # indicates p < 0.05 vs. LPS group; Δ indicates p < 0.05 vs. males LPS

Article Snippet: IL-1β, IL-6, TNF-α, CXCL10, and CCL2 in plasma and tissue homogenates were quantified using dual-antibody solid-phase immunoassays (ELISA Development Kit, Peprotech) according to the manufacturer's instructions.

Techniques: Expressing, Injection, Saline, Control, Enzyme-linked Immunosorbent Assay

β-FNAs effect on LPS-induced CXCL10, CCL2, IL-6, IL-1β, and TNF-α expression in the plasma of male and female C57BL/6J mice. Mice ( n = 11–12/group) were injected (i.p.) with saline (control), LPS (0.83 mg/kg), LPS followed immediately by β-FNA treatment (50 mg/kg; i.p.; LPS + β-FNA), or LPS followed by β-FNA 10 h post-LPS (LPS + β-FNA 10 h). 24 h post-LPS, mice were terminated followed by plasma collection. Levels of CXCL10 ( A ), CCL2 ( B ), IL-6 ( C ), IL-1β ( D ), and TNF-α ( E ) of plasma were measured by ELISA. Data are reported as mean ± SEM. Two-way ANOVA indicated a significant main effect of treatment ( p < 0.0001), sex ( p < 0.0001), and interaction ( p < 0.04) on CXCL10 levels in the plasma. Two-way ANOVA determined CCL2 had a significant main effect of sex ( p < 0.0001), treatment ( p < 0.0001), and interaction ( p < 0.0001). Two-way ANOVA determined IL-6 had a significant main effect of sex ( p < 0.0001) but not treatment ( p = 0.14) and interaction ( p = 0.06). Two-way ANOVA determined IL-1β had a significant main effect of sex ( p < 0.0001) but not treatment ( p = 0.28) or interaction ( p = 0.07). Two-way ANOVA determined TNF-α had a significant main effect of sex ( p < 0.04) and treatment ( p < 0.0001) but not interaction ( p = 0.08). Pairwise comparisons were assessed using a Fisher’s LSD test; * indicates p < 0.05 vs. saline group; # indicates p < 0.05 vs. LPS group; Δ indicates p < 0.05 vs. males LPS

Journal: Journal of Inflammation (London, England)

Article Title: Anti-inflammatory effects of β-FNA are sex-dependent in a pre-clinical model of LPS-induced inflammation

doi: 10.1186/s12950-023-00328-z

Figure Lengend Snippet: β-FNAs effect on LPS-induced CXCL10, CCL2, IL-6, IL-1β, and TNF-α expression in the plasma of male and female C57BL/6J mice. Mice ( n = 11–12/group) were injected (i.p.) with saline (control), LPS (0.83 mg/kg), LPS followed immediately by β-FNA treatment (50 mg/kg; i.p.; LPS + β-FNA), or LPS followed by β-FNA 10 h post-LPS (LPS + β-FNA 10 h). 24 h post-LPS, mice were terminated followed by plasma collection. Levels of CXCL10 ( A ), CCL2 ( B ), IL-6 ( C ), IL-1β ( D ), and TNF-α ( E ) of plasma were measured by ELISA. Data are reported as mean ± SEM. Two-way ANOVA indicated a significant main effect of treatment ( p < 0.0001), sex ( p < 0.0001), and interaction ( p < 0.04) on CXCL10 levels in the plasma. Two-way ANOVA determined CCL2 had a significant main effect of sex ( p < 0.0001), treatment ( p < 0.0001), and interaction ( p < 0.0001). Two-way ANOVA determined IL-6 had a significant main effect of sex ( p < 0.0001) but not treatment ( p = 0.14) and interaction ( p = 0.06). Two-way ANOVA determined IL-1β had a significant main effect of sex ( p < 0.0001) but not treatment ( p = 0.28) or interaction ( p = 0.07). Two-way ANOVA determined TNF-α had a significant main effect of sex ( p < 0.04) and treatment ( p < 0.0001) but not interaction ( p = 0.08). Pairwise comparisons were assessed using a Fisher’s LSD test; * indicates p < 0.05 vs. saline group; # indicates p < 0.05 vs. LPS group; Δ indicates p < 0.05 vs. males LPS

Article Snippet: IL-1β, IL-6, TNF-α, CXCL10, and CCL2 in plasma and tissue homogenates were quantified using dual-antibody solid-phase immunoassays (ELISA Development Kit, Peprotech) according to the manufacturer's instructions.

Techniques: Expressing, Clinical Proteomics, Injection, Saline, Control, Enzyme-linked Immunosorbent Assay

β-FNAs effect on LPS-induced CXCL10, CCL2, and TNF-α expression in the spleen of male and female C57BL/6J mice. Mice ( n = 11–12/group) were injected (i.p.) with saline (control), LPS (0.83 mg/kg), LPS followed immediately by β-FNA treatment (50 mg/kg; i.p.; LPS + β-FNA), or LPS followed by β-FNA 10 h post-LPS (LPS + β-FNA 10 h). 24 h post-LPS, mice were terminated followed by tissue collection. Levels of CXCL10 ( A ), CCL2 ( B ), and TNF-α ( C ) of tissue homogenates were measured by ELISA. Data are reported as mean ± SEM. Two-way ANOVA indicated a significant main effect of treatment ( p < 0.001), sex ( p < 0.0001), and interaction ( p < 0.001) on CXCL10 levels in the spleen. Two-way ANOVA determined CCL2 had a significant main effect of sex ( p < 0.0001), treatment ( p < 0.0001), and interaction ( p < 0.0001). Two-way ANOVA determined TNF-α had a significant main effect of treatment ( p < 0.003), but not sex ( p = 0.25) or interaction ( p = 0.54). Pairwise comparisons were assessed using a Fisher’s LSD test; * indicates p < 0.05 vs. saline group; # indicates p < 0.05 vs. LPS group; Δ indicates p < 0.05 vs. males LPS

Journal: Journal of Inflammation (London, England)

Article Title: Anti-inflammatory effects of β-FNA are sex-dependent in a pre-clinical model of LPS-induced inflammation

doi: 10.1186/s12950-023-00328-z

Figure Lengend Snippet: β-FNAs effect on LPS-induced CXCL10, CCL2, and TNF-α expression in the spleen of male and female C57BL/6J mice. Mice ( n = 11–12/group) were injected (i.p.) with saline (control), LPS (0.83 mg/kg), LPS followed immediately by β-FNA treatment (50 mg/kg; i.p.; LPS + β-FNA), or LPS followed by β-FNA 10 h post-LPS (LPS + β-FNA 10 h). 24 h post-LPS, mice were terminated followed by tissue collection. Levels of CXCL10 ( A ), CCL2 ( B ), and TNF-α ( C ) of tissue homogenates were measured by ELISA. Data are reported as mean ± SEM. Two-way ANOVA indicated a significant main effect of treatment ( p < 0.001), sex ( p < 0.0001), and interaction ( p < 0.001) on CXCL10 levels in the spleen. Two-way ANOVA determined CCL2 had a significant main effect of sex ( p < 0.0001), treatment ( p < 0.0001), and interaction ( p < 0.0001). Two-way ANOVA determined TNF-α had a significant main effect of treatment ( p < 0.003), but not sex ( p = 0.25) or interaction ( p = 0.54). Pairwise comparisons were assessed using a Fisher’s LSD test; * indicates p < 0.05 vs. saline group; # indicates p < 0.05 vs. LPS group; Δ indicates p < 0.05 vs. males LPS

Article Snippet: IL-1β, IL-6, TNF-α, CXCL10, and CCL2 in plasma and tissue homogenates were quantified using dual-antibody solid-phase immunoassays (ELISA Development Kit, Peprotech) according to the manufacturer's instructions.

Techniques: Expressing, Injection, Saline, Control, Enzyme-linked Immunosorbent Assay

β-FNAs effect on LPS-induced CXCL10, CCL2, and TNF-α expression in the liver of male and female C57BL/6J mice. Mice ( n = 11–12/group) were injected (i.p.) with saline (control), LPS (0.83 mg/kg), LPS followed immediately by β-FNA treatment (50 mg/kg; i.p.; LPS + β-FNA), or LPS followed by β-FNA 10 h post-LPS (LPS + β-FNA 10 h). 24 h post-LPS, mice were terminated followed by tissue collection. Levels of CXCL10 ( A ), CCL2 ( B ), and TNF-α ( C ) of tissue homogenates were measured by ELISA. Data are reported as mean ± SEM. Two-way ANOVA indicated a significant main effect of treatment ( p < 0.001) and sex ( p < 0.0001) on CXCL10 levels in the liver; but no significant effect on interaction ( p = 0.61). Two-way ANOVA determined CCL2 had a significant main effect of treatment ( p < 0.0001), but not of sex ( p = 0.25) or interaction ( p = 0.28). Two-way ANOVA determined TNF-α showed no main effects of sex ( p = 0.63), treatment ( p = 0.41), or interaction ( p = 0.99). Pairwise comparisons were assessed using a Fisher’s LSD test; * indicates p < 0.05 vs. saline group; # indicates p < 0.05 vs. LPS group; Δ indicates p < 0.05 vs. males LPS

Journal: Journal of Inflammation (London, England)

Article Title: Anti-inflammatory effects of β-FNA are sex-dependent in a pre-clinical model of LPS-induced inflammation

doi: 10.1186/s12950-023-00328-z

Figure Lengend Snippet: β-FNAs effect on LPS-induced CXCL10, CCL2, and TNF-α expression in the liver of male and female C57BL/6J mice. Mice ( n = 11–12/group) were injected (i.p.) with saline (control), LPS (0.83 mg/kg), LPS followed immediately by β-FNA treatment (50 mg/kg; i.p.; LPS + β-FNA), or LPS followed by β-FNA 10 h post-LPS (LPS + β-FNA 10 h). 24 h post-LPS, mice were terminated followed by tissue collection. Levels of CXCL10 ( A ), CCL2 ( B ), and TNF-α ( C ) of tissue homogenates were measured by ELISA. Data are reported as mean ± SEM. Two-way ANOVA indicated a significant main effect of treatment ( p < 0.001) and sex ( p < 0.0001) on CXCL10 levels in the liver; but no significant effect on interaction ( p = 0.61). Two-way ANOVA determined CCL2 had a significant main effect of treatment ( p < 0.0001), but not of sex ( p = 0.25) or interaction ( p = 0.28). Two-way ANOVA determined TNF-α showed no main effects of sex ( p = 0.63), treatment ( p = 0.41), or interaction ( p = 0.99). Pairwise comparisons were assessed using a Fisher’s LSD test; * indicates p < 0.05 vs. saline group; # indicates p < 0.05 vs. LPS group; Δ indicates p < 0.05 vs. males LPS

Article Snippet: IL-1β, IL-6, TNF-α, CXCL10, and CCL2 in plasma and tissue homogenates were quantified using dual-antibody solid-phase immunoassays (ELISA Development Kit, Peprotech) according to the manufacturer's instructions.

Techniques: Expressing, Injection, Saline, Control, Enzyme-linked Immunosorbent Assay

β-FNAs effect on LPS-induced CCL2 expression in the colon, proximal small intestine (PSI), and distal small intestine (DSI) of male and female C57BL/6J mice. Mice ( n = 11–12/group) were injected (i.p.) with saline (control), LPS (0.83 mg/kg), LPS followed immediately by β-FNA treatment (50 mg/kg; i.p.; LPS + β-FNA), or LPS followed by β-FNA 10 h post-LPS (LPS + β-FNA 10 h). 24 h post-LPS, mice were terminated followed by tissue collection. Levels of CCL2 of the colon ( A ), PSI ( B ), and DSI ( C ) of tissue homogenates were measured by ELISA. Data are reported as mean ± SEM. Two-way ANOVA indicated a significant main effect of treatment ( p < 0.0001), sex ( p < 0.001), and an interaction ( p < 0.002) on CCL2 levels in the colon. In the proximal small intestine two-way ANOVA determined CCL2 had a significant main effect of sex ( p < 0.001) and treatment ( p < 0.002) but not an interaction ( p = 0.13). In the distal small intestine two-way ANOVA determined CCL2 had a significant main effect of treatment ( p < 0.0001), but not sex ( p = 0.37) or interaction ( p = 0.42). Pairwise comparisons were assessed using a Fisher’s LSD test; * indicates p < 0.05 vs. saline group; # indicates p < 0.05 vs. LPS group; Δ indicates p < 0.05 vs. males LPS

Journal: Journal of Inflammation (London, England)

Article Title: Anti-inflammatory effects of β-FNA are sex-dependent in a pre-clinical model of LPS-induced inflammation

doi: 10.1186/s12950-023-00328-z

Figure Lengend Snippet: β-FNAs effect on LPS-induced CCL2 expression in the colon, proximal small intestine (PSI), and distal small intestine (DSI) of male and female C57BL/6J mice. Mice ( n = 11–12/group) were injected (i.p.) with saline (control), LPS (0.83 mg/kg), LPS followed immediately by β-FNA treatment (50 mg/kg; i.p.; LPS + β-FNA), or LPS followed by β-FNA 10 h post-LPS (LPS + β-FNA 10 h). 24 h post-LPS, mice were terminated followed by tissue collection. Levels of CCL2 of the colon ( A ), PSI ( B ), and DSI ( C ) of tissue homogenates were measured by ELISA. Data are reported as mean ± SEM. Two-way ANOVA indicated a significant main effect of treatment ( p < 0.0001), sex ( p < 0.001), and an interaction ( p < 0.002) on CCL2 levels in the colon. In the proximal small intestine two-way ANOVA determined CCL2 had a significant main effect of sex ( p < 0.001) and treatment ( p < 0.002) but not an interaction ( p = 0.13). In the distal small intestine two-way ANOVA determined CCL2 had a significant main effect of treatment ( p < 0.0001), but not sex ( p = 0.37) or interaction ( p = 0.42). Pairwise comparisons were assessed using a Fisher’s LSD test; * indicates p < 0.05 vs. saline group; # indicates p < 0.05 vs. LPS group; Δ indicates p < 0.05 vs. males LPS

Article Snippet: IL-1β, IL-6, TNF-α, CXCL10, and CCL2 in plasma and tissue homogenates were quantified using dual-antibody solid-phase immunoassays (ELISA Development Kit, Peprotech) according to the manufacturer's instructions.

Techniques: Expressing, Injection, Saline, Control, Enzyme-linked Immunosorbent Assay